transcriptional repressor of genes expressed in the transition phase
function
transition state regulator
product
transcriptional repressor ([SW|MarR family])
Genomic Context
categories
[category|SW 3|Information processing] → [category|SW 3.4|Regulation of gene expression] → [category|SW 3.4.2|Transcription factors and their control] → [category|SW 3.4.2.5|Transcription factors/ other][category|SW 3|Information processing] → [category|SW 3.4|Regulation of gene expression] → [category|SW 3.4.6|Transition state regulators][category|SW 6|Groups of genes] → [category|SW 6.4|Phosphoproteins] → [category|SW 6.4.1|Phosphorylation on an Arg residue]Gene
Coordinates
1,073,106 → 1,073,717
The protein
[SW|Domains]
[SW|HTH marR-type domain] (aa 13-157) (according to UniProt)Modification
phosphorylated on Arg-3 [Pubmed|22517742]Effectors of protein activity
activity is inhibited in an unknown way by [protein|83F1BE86A7AE1098C1319198A8DDA6B8C5F49236|PrkA] (possibly phosphorylation) [Pubmed|25983726]Structure
[PDB|2FXA]additional information
information on binding sites can be found in the [http://www.prodoric2.de/detail.php?acc=MX000050 PRODORIC2 database]Expression and Regulation
Operons
genes
[gene|F7FD94CE7AB290FBFD3A9B88F204961668F9B42C|scoC]
description
[Pubmed|3131303]
sigma factors
[protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA]: sigma factor, [Pubmed|19251843], in [regulon|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA regulon]regulatory mechanism
[protein|E5110D9C36E8C55AFD1419987B14A53232165F20|AbrB]: activation, [Pubmed|2504584], in [regulon|E5110D9C36E8C55AFD1419987B14A53232165F20|AbrB regulon][protein|69D55899C7CE30A282AF6011527FAD55A76CDBDA|SenS]: positive regulation, in [regulon|69D55899C7CE30A282AF6011527FAD55A76CDBDA|SenS regulon][protein|8841E35B7A90F6716C0BCD98E8D0CC05FE1CA471|SalA]: negative regulation, in [regulon|8841E35B7A90F6716C0BCD98E8D0CC05FE1CA471|SalA regulon][protein|90ACE0DECA58D3C1A55E135120F7A0C1C920571C|CodY]: negative regulation, in [regulon|90ACE0DECA58D3C1A55E135120F7A0C1C920571C|CodY regulon]regulation
expressed upon transition into the stationary phase ([protein|search|AbrB]) [Pubmed|2504584]view in new tabBiological materials
Mutant
1A918 ( ''scoC''::''erm trpC2 leuC7''), [Pubmed|15126467], available at the [http://bgsc.org/getdetail.php?bgscid=1A918 Bacillus Genetic Stock Center]BKE09990 (Δ[gene|F7FD94CE7AB290FBFD3A9B88F204961668F9B42C|scoC]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE09990 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTACGTCACCTGCTTCTC, downstream forward: _UP4_GAAGAGCTCGAACCTGTAAABKK09990 (Δ[gene|F7FD94CE7AB290FBFD3A9B88F204961668F9B42C|scoC]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK09990 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTACGTCACCTGCTTCTC, downstream forward: _UP4_GAAGAGCTCGAACCTGTAAAExpression vectors
for expression, purification in E. coli with N-terminal His-tag, pRSETA available in Gerth labReferences
Reviews
Original publications
3131303,16923912,1906467,10383984,16321961,14629015,1907636,15126467,2504584,15104138,19118355,19801406,19898538,20382764,22517742,23660663,11717292,25966844,19251843,26473603,25983726,26728191,31039790